LOCUS       B4GALNT4-pGEn2          8316 bp    DNA     circular     20-SEP-2016
FEATURES             Location/Qualifiers
     CDS             129..1108
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_recomb     complement(3839..3867)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    6944..7485
                     /note="WPRE"
     misc_recomb     6906..6928
                     /label=attB2
                     /note="attB2 site"
                     /note="recognized as attB2"
     misc_feature    complement(7492..7706)
                     /note="BGH pA"
     misc_feature    complement(7794..7923)
                     /note="3'-ITR"
     misc_feature    2982..3056
                     /note="TCM signal sequence"
     misc_feature    3060..3083
                     /note="His tag"
     misc_feature    3084..3134
                     /note="AviTag"
     misc_feature    3135..3830
                     /note="Superfolder GFP"
     misc_feature    2976..2981
                     /note="Kozak seq"
     misc_feature    3868..3890
                     /note="TEV site"
     misc_feature    3891..6899
                     /note="OPTIMIZED B4GALNT4 coding region"
     vector          join(6914..8316,1..3857)
                     /source="pGEn2-DEST"
                     /type="Destination Vector"
     clone           3858..6913
                     /source="B4GALNT4 pDONR"
                     /type="Entry Clone"
     source          1..8316
                     /dnas_title="B4GALNT4-pGEn2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC
     3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC
     3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA
     3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG
     3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG
     3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC
     3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG
     3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC
     3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG
     3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA
     3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA
     3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG
     3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
     3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG
     3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT
     3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc AGACAGGGCA
     3901 GAGCCCTGCG GCAGAGACTG GGATATGGAA GAGATGGCGA GAAGCTGACC AGCGAGACAG
     3961 ATGGAAGGGG TGTCCATGCT GCCCCTTCTA CACAGAGAGC CGAGGATAGC AGCGAGAGCA
     4021 GGGAAGAAGA ACAGGCCCCT GAGGGCAGGG ACCTGGATAT GCTGTTTCCT GGCGGAGCTG
     4081 GCAGACTGCC CCTGAATTTC ACACACCAGA CCCCTCCATG GCGGGAAGAG TATAAGGGAC
     4141 AGGTCAACCT GCACGTGTTC GAGGATTGGT GCGGAGGTGC TGTGGGCCAC CTGAGAAGAA
     4201 ATCTGCACTT CCCCCTGTTC CCCCACACCC GGACAACAGT GAAAAAGCTG GCCGTGTCCC
     4261 CCAAGTGGAA GAACTACGGC CTGCGGATCT TCGGCTTTAT CCACCCTGCT AGAGATGGGG
     4321 ACGTGCAGTT TAGCGTGGCC AGCGACGATA ACAGCGAGTT CTGGCTGAGC CTGGATGAAT
     4381 CTCCTGCTGC TGCCCAGCTG GTGGCCTTTG TGGGAAAGAC AGGATCTGAA TGGACCGCCC
     4441 CTGGCGAGTT CACCAAGTTT AGCAGCCAGG TGTCCAAGCC CAGACGGCTG ATGGCCAGCA
     4501 GAAGGTACTA CTTCGAGCTG CTGCACAAGC AGGACGACAG AGGCAGCGAT CATGTGGAAG
     4561 TGGGATGGCG GGCTTTTCTG CCCGGCCTGA AGTTTGAAGT GATCAGCAGC GCCCACATCA
     4621 GCCTGTACAC AGATGAGAGC GCCCTGAAGA TGGATCACGT GGCCCATGTG CCTCAGTCTC
     4681 CAGCATCTCA TGTGGGAGGC AGACCCCCTC AGGAAGAGAC ATCTGCCGAC ATGCTGAGGC
     4741 CCGACCCCAG AGATACCTTT TTCCTGACCC CTCGGATGGA AAGCAGCAGC CTGGAAAACG
     4801 TGCTGGAACC TTGTGCCTAC GCCCCCACCT ACGTGGTCAA GGATTTCCCT ATCGCCAGAT
     4861 ACCAGGGCCT GCAGTTCGTG TACCTGAGCT TTGTGTACCC CAACGACTAC ACCCGGCTGA
     4921 CCCACATGGA AACCGACAAC AAGTGCTTCT ACCGCGAGAG CCCCCTGTAC CTGGAAAGAT
     4981 TCGGCTTCTA CAAGTACATG AAAATGGACA AAGAGGAAGG CGACGAGGAC GAGGAAGATG
     5041 AGGTCCAGAG AAGGGCCTTC CTGTTCCTGA ACCCCGACGA CTTCCTGGAC GATGAGGATG
     5101 AAGGCGAACT GCTGGATTCC CTGGAACCCA CAGAAGCCGC TCCTCCAAGA TCTGGACCAC
     5161 AGAGTCCTGC TCCAGCTGCT CCTGCTCAGC CTGGCGCTAC ACTGGCTCCA CCTACACCAC
     5221 CTAGACCTAG GGACGGCGGA ACACCAAGAC ACAGCAGAGC ACTGTCTTGG GCCGCTAGAG
     5281 CTGCTAGACC TCTGCCACTG TTTCTGGGAA GGGCACCTCC TCCCAGACCT GCTGTGGAAC
     5341 AGCCACCTCC TAAGGTGTAC GTGACAAGAG TGCGACCTGG CCAGAGAGCT TCTCCTAGAG
     5401 CACCAGCACC AAGAGCCCCT TGGCCTCCTT TTCCTGGTGT CTTTCTGCAC CCCAGACCAC
     5461 TGCCTAGAGT CCAGCTGAGA GCACCCCCAA GACCTCCAAG GCCTCATGGT AGAAGAACTG
     5521 GCGGACCTCA GGCCACACAG CCAAGACCAC CCGCAAGAGC ACAGGCTACA CAGGGCGGAA
     5581 GAGAAGGCCA GGCTAGAACA CTGGGACCTG CCGCTCCAAC CGTGGATAGC AATCTGTCTA
     5641 GCGAGGCCAG ACCCGTGACC AGCTTTCTGA GTCTGTCTCA GGTCAGCGGT CCTCAGCTGC
     5701 CTGGCGAAGG GGAAGAGGAG GAAGAAGGGG AGGACGACGG CGCTCCTGGG GATGAAGCTG
     5761 CTTCTGAGGA TTCTGAAGAG GCCGCTGGAC CAGCTCTCGG AAGGTGGCGC GAAGATGCCA
     5821 TCGATTGGCA GAGAACATTT TCCGTGGGCG CCGTGGACTT CGAACTCCTG AGATCCGACT
     5881 GGAACGACCT GAGGTGCAAC GTGTCAGGCA ATCTGCAGCT GCCAGAGGCC GAAGCTGTGG
     5941 ATGTGACAGC CCAGTACATG GAACGGCTGA ATGCCAGACA CGGCGGCAGA TTTGCCCTGC
     6001 TGAGAATCGT GAACGTGGAA AAGCGGAGAG ACAGCGCTAG AGGCTCCAGA TTCCTCCTGG
     6061 AACTGGAACT CCAGGAAAGA GGCGGCGGAA GGCTGAGACT GTCTGAGTAT GTGTTTCTGA
     6121 GGCTGCCAGG CGCCAGAGTG GGAGATGCCG ATGGCGAATC TCCAGAACCA GCTCCTGCAG
     6181 CCAGCGTCAG ACCAGATGGT AGACCTGAAC TGTGTAGGCC TCTGCGGCTG GCTTGGAGAC
     6241 AGGACGTGAT GGTGCACTTC ATCGTGCCCG TGAAAAACCA GGCCAGATGG GTGGCACAGT
     6301 TCCTGGCAGA TATGGCCGCT CTGCATGCCA GAACCGGCGA TTCTAGATTC AGCGTGGTCC
     6361 TGGTGGATTT CGAGAGCGAG GACATGGATG TGGAAAGGGC CCTGAGAGCC GCAAGACTGC
     6421 CAAGATATCA GTACCTGCGG CGGACCGGCA ACTTTGAAAG ATCTGCTGGG CTCCAGGCAG
     6481 GCGTGGACGC TGTCGAGGAT GCCTCTTCTA TCGTGTTCCT GTGCGATCTG CACATTCACT
     6541 TCCCTCCCAA CATTCTGGAC GGCATCCGGA AGCACTGTGT GGAAGGCAGG CTGGCATTTG
     6601 CCCCTGTGGT CATGAGGCTG TCTTGTGGCA GCTCCCCTAG AGATCCTCAC GGCTACTGGG
     6661 AAGTCAACGG CTTCGGACTG TTTGGCATCT ACAAGAGCGA CTTCGATAGA GTGGGCGGCA
     6721 TGAACACCGA GGAATTCAGA GATCAGTGGG GAGGCGAGGA CTGGGAACTG CTCGACAGAG
     6781 TGCTGCAGGC AGGGCTGGAA GTCGAGAGGC TCAGACTGCG GAATTTCTAC CACCACTACC
     6841 ACAGCAAGCG GGGCATGTGG TCCGTCAGAT CTAGAAAGGG ATCTAGGACC GGCGCCAGCT
     6901 AGGACCCAGC TTTCTTGTAC AAAGTGGTTG ATAGCTTGGA TCCAATCAAC CTCTGGATTA
     6961 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG
     7021 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC
     7081 CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA
     7141 ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC
     7201 CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT
     7261 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC
     7321 CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG
     7381 GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC
     7441 TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGAGATC TGCCTCGACT
     7501 GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG
     7561 GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG
     7621 AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG
     7681 GAAGACAATA GCAGGCATGC TGGGGACTCG AGTTAAGGGC GAATTGCCGA TTAGGATCTT
     7741 CCTAGAGCAT GGCTACGTAG ATAAGTAGCA TGGCGGGTTA ATCATTAACT ACAAGGAACC
     7801 CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG
     7861 ACCAAAGGTC GCCCGACGCC CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG
     7921 CAGCCTTAAT TAAGAGCTCA TGGCGCGCCT AGGCCTTGAC GGCCTTCCGC CAATTCGCCC
     7981 TATAGTGAGT CGTATTACGT CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA
     8041 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG
     8101 TAATAGCGAA GAGGCCCGCA CCGAAACGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG
     8161 AATGGGAGCG CCCTGTAGCG GCCACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA
     8221 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC
     8281 GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAG
//

